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KMID : 0616120220530040181
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Korean Journal of Pharmacognosy
2022 Volume.53 No. 4 p.181 ~ p.191
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Esculetin Suppresses the Growth and Proliferation of A431 Skin Cancer Cells via the MAPKs Pathway
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Sung Jin-Young
Kim Yong-Min
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Abstract
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As the incidence of skin cancer increases every year, non-surgical treatment methods for cancer are being sought. Esculetin, a natural dihydroxy coumarin, is attracting attention as a therapeutic agent for certain diseases, such as cancer, based on its broad pharmacological activity. In this study, the anticancer ability of esculetin was evaluated using the epidermoid carcinoma cell line A431. As a result of evaluating the apoptosis ability of esculetin by MTT assay, apoptosis was observed in a time-concentration-dependent manner regardless of the presence or absence of FBS. As a result of quantitative real-time PCR, esculetin reduced cyclin D1 mRNA in a time-concentration-dependent manner. In addition, as a result of western blotting, esculetin significantly inhibited phosphorylation of ERK, JNK, and p38 in a concentration-dependent manner. The results of this study suggest that esculetin has the potential to be used as an effective natural medicine for the treatment of skin cancer.
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KEYWORD
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Esculetin, Anti-cancer, Cell cycle, Apoptosis, Mitogen-activated protein kinases
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